ISOLATION OF WHITE SPOT SYNDROME VIRUS (WSSV) IN EGYPTIAN SHRIMP USING CONVENTIONAL PCR AND REAL TIME PCR (QPCR) TECHNIQUES
نویسندگان
چکیده
منابع مشابه
Design and Evaluation of a PCR Method for Detecting White Spot Syndrome Virus in Shrimp
Background: White spot syndrome virus (wssv) is the causing agent for white spot disease in shrimp and many crustaceans. This disease is highly contagious and can cause death within 3–10 days under normal culture conditions. Therefore, early diagnosis of the virus is a necessity. Materials and Methods: Primers were designed for three regions of the virus genome and one region of the shrimp geno...
متن کاملEfficiency and sensitivity determination of Shrimple®, an immunochromatographic assay for white spot syndrome virus (WSSV), using quantitative real-time PCR
White spot syndrome virus (WSSV) is a prevalent and virulent pathogen affecting both wild and cultured penaeid shrimp worldwide. Molecular diagnostic tools have made detection of the virus increasingly accurate. However, these techniques are often not readily available for rapid diagnosis in the field or in shrimp production facilities. Shrimple®, an immunochromatographic detection assay for WS...
متن کاملImmunization of Litopenaeus vannamei shrimp against white spot syndrome virus (WSSV) by gamma-irradiated WSSV plus Vibrio paraheomolyticus
Introduction: White spot syndrome virus (WSSV) is one of the most deadly infectious pathogens of the shrimp culture industry. Neither effective vaccines nor efficient treatments are currently available for this disease. Vibrio species are well known dominant bacterial pathogens in the shrimp ponds. As facultative pathogenic bacteria, it is possible that Vibrio spp. along with WSSV to co-infect ...
متن کاملIncreasing production in Korean shrimp farms with white-spot syndrome virus PCR-negative brood stock.
White-spot syndrome virus (WSSV) is a devastating, infectious virus affecting shrimp. Although sensitive techniques involving PCR have been developed to assist farmers in screening shrimp (brood stock) for WSSV prior to stocking ponds, such practices have not yet been applied in Korea. Despite the rationality of implementing screening, there has been some doubt as to whether the stocking of WSS...
متن کاملSequence analysis of ORF94 in different White Spot Syndrome Virus (WSSV) isolates of Iran
White spot syndrome virus (WSSV) is a pathogen that causes high mortality in shrimp culture in the whole world. Sequence analysis of WSSV has shown similarity of WSSV isolates in different countries with exception of a few variable genomic loci. This study investigated the sequence variation of some Iranian WSSV isolates and previously identified isolates. Samples were collected during target ...
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ژورنال
عنوان ژورنال: SLOVENIAN VETERINARY RESEARCH
سال: 2019
ISSN: 1580-4003
DOI: 10.26873/svr-783-2019